Intermediate

Part:BBa_K933002

Designed by: Hannah Jepsen-Burger   Group: iGEM12_Penn_State   (2012-10-01)


short leader sequence with mCherry and GFP reporters

This sequence has the available space to insert repeating codon sequences in an effort to determine codon optimization in E.coli. It is prefaced by a weak RBS, a short leader sequence, and contains restriction enzyme sites that allow for the insertion of the repeat sequences. Two reporters-mCherry and GFP-show the degradation of tRNA as the repeated sequences interfere with natural translation. mCherry should change with changing sequences, and GFP should remain constant.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 71
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 71
    Illegal NheI site found at 99
    Illegal NheI site found at 122
    Illegal NotI site found at 77
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 71
    Illegal BamHI site found at 202
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 71
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 71
    Illegal XbaI site found at 86
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None